Exosome Preparation Services
BOC Sciences specializes in exosome preparation services, delivering high-purity, application-driven exosome materials for use in pharmaceutical development, regenerative medicine, and cosmetic innovation. Our comprehensive workflows include selective cell culture optimization, conditioned medium harvesting, and multistep purification technologies such as ultracentrifugation, tangential flow filtration (TFF), and size-exclusion chromatography. Each batch is prepared under GMP-compliant conditions to ensure particle uniformity, membrane integrity, and biological activity. We provide full customization based on source cells (e.g., MSCs, immune cells, tumor cells) and target applications, ranging from drug delivery and immune modulation to anti-aging and skin regeneration. BOC Sciences supports both research-scale and commercial-scale exosome production, enabling innovation and product reliability across research, therapeutic, and cosmetic pipelines.
Key Advantages of High-Quality Exosome Preparation Services
High Purity and Bioactivity
Optimized purification methods ensure exosomes retain their functional proteins, RNAs, and membranes.
Diverse and Customizable Cell Sources
Exosomes are derived from various cell types, offering tailored solutions for diverse applications.
Rigorous Quality Control System
Comprehensive assays ensure batch-to-batch consistency, including size distribution, imaging, and marker verification.
Animal-Free, GMP-Compliant Processing
Our exosome production system is entirely free of serum and animal-derived components, significantly reducing the risk of biological impurities.
Scalable Manufacturing Capability
From research-grade to commercial scale, production processes are adaptable to meet increasing demands.
Broad Application Compatibility
Exosomes support drug delivery, wound healing, anti-aging therapy, and tissue regeneration.
Excellent Structural and Membrane Integrity
Cold-chain and optimized protocols maintain exosomal membrane stability for enhanced delivery efficacy.
Integrated Technical Support and Custom Development
Full-spectrum support from cell culture optimization to exosome formulation accelerates R&D and product development.
Premium Exosome for Medical, Aesthetic, and Research Applications by BOC Sciences
Our company offers a range of exosome-based products designed for advanced medical and aesthetic treatments. These exosomes are sourced from various biological origins, each bringing unique benefits for promoting skin health, wound healing, tissue regeneration, and anti-aging effects. Here is a detailed portfolio of the exosome products we offer:
Umbilical MSC-Derived Exosomes
Sourced from umbilical mesenchymal stem cells, these exosomes are rich in growth factors and cytokines, promoting skin cell proliferation, differentiation, and repair. They are particularly useful in anti-aging and wound healing treatments.
Adipose Stem Cell-Derived Exosomes
Derived from adipose stem cells, these exosomes regulate tissue metabolism, vascular remodeling, and muscle function. They are commonly used in the aesthetic industry to improve skin condition and promote tissue regeneration.
Synthetic Exosomes
Created through emulsification techniques and charge-mediated vesicle assembly, synthetic exosomes can carry small nucleic acids like miRNA and be surface-modified with target proteins. They are suitable for research on cell signaling pathways and the development of cosmetic products.
Milk-Derived Exosomes
Extracted from cow's milk, these exosomes possess significant biological activity. They are used in the development of functional skincare products, offering benefits such as hydration and antioxidant effects.
Engineered Exosomes
These exosomes are produced by modifying parental cells or directly engineering natural exosomes, allowing for efficient and rapid production of large quantities. They are ideal for targeted medical and aesthetic treatments.
Skin Stem Cell-Derived Exosomes
Sourced from skin stem cells, these exosomes help promote the self-repair and regeneration of the skin, making them valuable in treatments for skin damage, skin rejuvenation, and overall anti-aging effects.
Peripheral Blood-Derived Exosomes
Extracted from peripheral blood, these exosomes contain a variety of secreted exosomes from different cells. They are useful in disease monitoring and serve as tools for studying the physiological and pathological mechanisms of the skin.
iPSC-Derived Exosomes
Derived from induced pluripotent stem cells (iPSCs), these exosomes exhibit properties similar to embryonic stem cells. They are used in regenerative medicine and anti-aging treatments, promoting tissue repair and regeneration.
Cutting-Edge Exosome Preparation Technologies Offered by BOC Sciences
BOC Sciences employs advanced exosome isolation and purification technologies to ensure high-quality, bioactive extracellular vesicles for medical, aesthetic, and research use. Our standardized workflows combine cutting-edge separation techniques with rigorous quality control to deliver exosomes of high purity, integrity, and functional activity. We support scalable, GMP-compliant production and offer customizable solutions to meet specific downstream application needs. Below is an overview of our exosome preparation technologies:
Tangential Flow Filtration (TFF)
TFF offers scalable concentration and diafiltration of exosomes while preserving morphology and function, making it ideal for both upstream processing and downstream formulation in therapeutic and diagnostic applications.
Ultracentrifugation
This density-based separation technique uses high centrifugal forces to pellet exosomes, enabling initial enrichment from complex matrices, though it may compromise purity and require long processing times and specialized equipment.
Size-Exclusion Chromatography (SEC)
SEC gently separates exosomes from proteins and smaller molecules based on hydrodynamic radius, maintaining exosome integrity and yielding high-purity fractions suitable for downstream bioanalytical or functional assays.
Immunoaffinity Capture
Leverages antibodies against exosomal surface antigens to selectively isolate defined subpopulations, enabling targeted analysis or therapeutic loading, with enhanced specificity and minimal background interference.
Microfluidic Separation
Microfluidic systems integrate size, charge, or affinity-based isolation in compact devices, supporting rapid, high-resolution exosome capture with minimal sample input—ideal for diagnostic platforms and translational research.
Enzyme-Free Lysis and Debris Removal
Applies mechanical or mild chemical strategies for cell disruption and debris clearance, reducing enzymatic contamination risks while preserving exosome architecture and surface protein profiles for downstream applications.
GMP-Compliant Exosome Manufacturing Capabilities
We have established well-validated processes for exosome extraction and purification, ensuring consistent, high-quality production for research and industrial applications. Our facilities comply with GMP standards, featuring scalable production lines and precision instrumentation to maintain high quality across different batch sizes. Whether for small-volume R&D batches or large-scale production, we offer flexible and traceable manufacturing solutions. Our facilities include ISO-classified cleanrooms, automated filtration and purification systems, and in-house QC laboratories to monitor critical quality attributes such as particle size, surface markers, sterility, and endotoxin levels.
Standardized and Reliable Exosome Quality Control Testing
Quality assurance is a fundamental aspect of exosome production, directly impacting their safety, consistency, and functional performance. BOC Sciences offers comprehensive exosome quality testing services to evaluate critical quality attributes throughout the entire production process. From raw material input to final exosome preparations, our workflow incorporates advanced analytical technologies to assess identity, purity, particle characteristics, biological activity, and stability. These services support diverse application needs across research, cosmetics, diagnostics, and biotechnology, ensuring each batch delivers reliable and reproducible results for downstream use.
Testing Instruments
Nanoparticle Tracking Analysis (NTA) System
Transmission Electron Microscope (TEM)
Dynamic Light Scattering (DLS) Instrument
Tunable Resistive Pulse Sensing (TRPS) Analyzer
Flow Cytometer (FACS)
Western Blotting System
Enzyme-Linked Immunosorbent Assay (ELISA) Reader
Real-Time PCR System
Endotoxin Testing System
Spectrophotometer (UV-Vis/NIR)
Analytical Items
Particle Size and Concentration Analysis
Exosome Marker Profiling
Morphology Characterization (TEM)
Protein and RNA Quantification
Surface Protein Expression
Endotoxin Testing (LAL Assay)
Sterility and Microbial Limit Testing
pH and Osmolality Measurement
Stability and Storage Condition Testing
Residual Impurity Analysis
What Applications Does Exosome Preparation Support in Biomedical and Aesthetic Fields?
Exosomes, naturally occurring extracellular vesicles, have shown great promise in both biomedical and aesthetic applications due to their ability to deliver bioactive molecules, promote cellular regeneration, and improve tissue function.
Anti-Aging Treatment
Exosomes promote skin cell repair, enhance collagen synthesis, and strengthen the skin barrier, helping to combat aging by reducing fine lines and wrinkles, improving skin texture, and boosting elasticity.
Skin Repair and Regeneration
Exosomes stimulate skin cell regeneration and healing, making them ideal for improving acne scars, hyperpigmentation, and promoting overall skin rejuvenation, enhancing texture and elasticity.
Hair Regeneration
Exosomes stimulate hair follicle growth and proliferation, promoting new hair growth and effectively addressing issues like male-pattern baldness and alopecia areata.
Whitening
Exosomes control pigment metabolism and block melanin formation, which helps to create an even skin tone and bright complexion while reducing hyperpigmentation to give a more youthful appearance.
Cancer Targeted Therapy
Exosomes can serve as carriers for drugs or therapeutic RNA, precisely delivering anticancer agents to tumor sites, reducing side effects on healthy tissues, and enhancing treatment efficacy.
Gene Therapy
Exosomes are used to deliver DNA, mRNA, or CRISPR-Cas9 gene editing tools, assisting in repairing genetic mutations or treating inherited diseases, providing a promising approach to genetic therapies.
Immunotherapy
Exosomes act as vaccine carriers, delivering pathogen information to activate immune responses, enhancing cancer treatment, and offering potential solutions for viral infections.
Neurodegenerative Disease Treatment
Exosomes contain neurotrophic factors that protect neural cells, holding therapeutic potential for treating neurodegenerative diseases such as Alzheimer's and Parkinson's, and improving cognitive function.
FAQs
What Are the Active Ingredients in Exosomes?
Exosomes contain a variety of bioactive molecules that are crucial for their therapeutic effects. These include proteins, lipids, RNA (such as mRNA, microRNA), and metabolites. The protein content of exosomes typically includes surface markers (like CD9, CD63, and CD81), signaling molecules, and enzymes involved in cellular communication and regeneration. The RNA molecules play a key role in gene regulation and cellular responses, while lipids contribute to membrane stability and functionality.
What Are Exosomes Made Of?
Exosomes are tiny extracellular vesicles that consist mainly of a lipid bilayer membrane along with proteins and nucleic acids. The exosome membrane contains high levels of phospholipids and cholesterol that serve to safeguard its internal cargo. The proteins found in exosomes include membrane proteins, signaling molecules, and enzymes, while the genetic material contained within exosomes primarily consists of mRNA, microRNA, and occasionally DNA. These components enable exosomes to participate in intercellular communication and mediate various biological functions.
How Are Exosomes Prepared for Use in Therapeutic Applications?
Exosomes are typically isolated from cell cultures or biological fluids such as blood, urine, or ascitic fluid. The preparation process involves several steps: collection of the exosome-containing fluid, isolation through methods like ultracentrifugation or size-exclusion chromatography, and purification to remove contaminants. After isolation, the exosomes can be characterized for size, purity, and biological activity before being used in therapeutic or cosmetic applications. These preparation steps ensure that the exosomes are safe and effective for use.
What Are the Benefits of Using Exosomes in Regenerative Medicine?
Exosomes are gaining significant attention in regenerative medicine because they are rich in growth factors, cytokines, and other bioactive molecules that promote tissue repair and regeneration. When applied to damaged tissues, exosomes can stimulate cellular regeneration, reduce inflammation, and enhance wound healing. Their ability to transfer molecular signals between cells makes them an ideal candidate for treating conditions such as heart disease, neurodegenerative disorders, and musculoskeletal injuries, offering a promising alternative to traditional stem cell therapies.