Protein-based Nanoparticle Drug Delivery System Development

BOC Sciences is dedicated to innovative formulations for nanoparticle drug delivery systems. We provide protein nanoparticle drug delivery system development services, relying on the unique properties of protein nanoparticles, carefully designed and implemented targeted, controllable and efficient drug delivery strategies, bringing revolutionary breakthroughs to modern healthcare.

Advantages of protein-based nanoparticle

Protein, a multifunctional biological macromolecule in nature, is known for its rich diversity and unique structural motif, which provides an ideal carrier choice for drug delivery. Their controllable modification capabilities and self-assembly properties allow proteins to build nanoparticles with precise properties that are perfectly suited to a variety of drug delivery needs. Protein nanoparticle drug delivery system skillfully integrates the essence of animal, plant or recombinant protein, and synergies with drugs to build a nanoparticle drug delivery system with excellent biocompatibility, reliable biodegradability, low antigenicity, high stability and powerful drug loading capacity. This system has shown unprecedented therapeutic potential and important value in clinical treatment, especially in key areas such as tumor targeted therapy.

Fig. 1 Delivery of protein nanoparticle to the cell. Intracellular delivery of insoluble drugs by proteinnanoparticles via the endocytosis process. (Hong, S., 2020)

The core advantages demonstrated by protein-based nanoparticles further highlight their unique position in the field of drug delivery:

Biocompatibility: The intrinsic biocompatibility of protein nanoparticles ensures a very low risk of toxicity and immune reactions, providing patients with a safe and gentle treatment experience and reducing adverse reactions during treatment.

Biodegradability: The inherent enzyme system in the body can efficiently degrade protein nanoparticles, effectively prevent the long-term accumulation of residual substances in the body, ensure the safety and biocompatibility of treatment, and avoid the possible side effects of long-term treatment.

Targeted delivery: Through precise functional design, such as the modification of ligands, antibodies or peptides, protein nanoparticles can be accurately located to the target cell or tissue, significantly improving the therapeutic effect of drugs, while minimizing the impact on non-target sites, reducing off-target effects, and improving the accuracy and safety of therapy.

Controlled release: The protein nanoparticle system is cleverly designed to achieve controlled release of drugs, ensuring a constant effective concentration of therapeutic drugs in the body, extending the duration of drug action, reducing the frequency of administration, and improving patient quality of life and treatment compliance.

Versatility: The diversity of proteins gives nanoparticles the potential for customization. By flexibly adjusting particle size, surface charge and drug loading characteristics, the nanoparticles can meet complex and variable therapeutic needs and enhance the personalization and precision of treatment regimens.

Enhanced stability: Protein nanoparticles can effectively protect encapsulated drugs from enzymatic degradation and other external factors, improve the stability and bioavailability of drugs, extend the shelf life of drugs, and ensure the quality of drugs during storage and transportation.

Protein-based nanoparticle applications

Cancer treatment: In the field of cancer treatment, protein-based nanoparticles have become an important part of precision medicine. They enable precise, targeted delivery of chemotherapy drugs to tumor cells while minimizing damage to healthy tissue.

Chronic disease management: For chronic diseases such as diabetes and rheumatoid arthritis, protein-based nanoparticles improve patients' quality of life and treatment compliance through continuous, precise drug delivery mechanisms. Gelatin-based nanoparticles, for example, have been successfully applied to the long-acting delivery of insulin and anti-inflammatory drugs, providing new ideas for long-term disease management and demonstrating great potential in improving patients' quality of life.

Vaccine delivery: Protein-based nanoparticles play an important role in vaccine development, as they can act as efficient carriers, accurately display antigens, stimulate the body's immune response, and thus enhance the immune effectiveness of vaccines. This delivery method not only improves the safety and effectiveness of vaccines, but also provides a more flexible and innovative platform for vaccine development.

Gene therapy: In the field of gene therapy, protein nanoparticles can encapsulate genetic material such as DNA or RNA for gene editing or gene silencing therapy. This technology opens up a new path for the treatment of refractory diseases such as genetic diseases and cancer, and is expected to fundamentally solve the root causes of diseases and bring the hope of cure to patients.

Our protein-based nanoparticle development services

Design and selection of protein carrier

In the early stages of development, we follow rigorous scientific principles to carefully design and select the most suitable protein carrier. Our team of experts selects the best carrier from a wide range of natural and synthetic proteins, such as albumin, casein, fibroin, according to the therapeutic load and application requirements to ensure optimal performance in the next steps.

Protein nanoparticle formulation

The formulation stage is a key part of the entire development process, where we use advanced engineering techniques including emulsification, dissolving, coagulation, and nanoprecipitation to refine selected protein carriers into nanoparticles with ideal size, shape, and surface properties. The laboratory is equipped with advanced instruments such as homogenizer, ultrasonic processor, freeze dryer, spray dryer, microfluidic device and high speed centrifuge to ensure the high quality output of nanoparticles.

Drug encapsulation and charging

We use original technology to ensure the high drug loading efficiency and stability of the therapeutic agent in the nanoparticles, by optimizing pH, ionic strength, solvent conditions and other parameters, to achieve the perfect synergy between the drug and the nanoparticles, thereby improving the bioavailability and therapeutic effect of the therapeutic agent.

Surface modification and targeting of protein nanoparticles

To achieve precise drug delivery, we use innovative ligand coupling strategies such as biotin-avidin bridging systems, click chemistry, photosensitive coupling techniques, pegylation, etc., to attach targeted molecules such as antibodies, peptides or small molecules to the surface of the nanoparticle, allowing it to bind specifically to the target cell or tissue. This customized design not only enhances the therapeutic effect, but also minimizes the drug exposure in non-target areas, improving the accuracy and safety of treatment.

Characterization and evaluation of protein nanoparticles

Comprehensive characterization of protein-based nanoparticles is key to ensuring their function and stability. We use a range of advanced analytical techniques, including:

Dynamic light scattering (DLS): The precise determination of the size and distribution of nanoparticles ensures their efficient delivery in the body.

Zeta potential analysis: Assessing the surface charge and stability of nanoparticles, which is critical for their behavior in a biological environment.

Transmission electron microscopy (TEM): provides high-resolution images of nanoparticles for in-depth analysis of their morphology and structure.

Encapsulation efficiency and drug release research: Combined with modern analytical techniques such as high performance liquid chromatography (HPLC) and mass spectrometry (MS), the encapsulation efficiency and release kinetics of drugs were accurately determined.

Advantages of our services

• Advanced technology
• First-class equipment
• Professional team
• Customized services
• One-stop service

Our workflow

• Demand analysis and formulation design
• Preparation and optimization of protein nanoparticles
• Study on drug encapsulation and release
• Biocompatibility and safety assessment
• Study on pharmacodynamics and pharmacokinetics
• Data analysis and reporting

In conclusion, protein-based nanoparticles are increasingly being used in biomedical fields, from cancer treatment to chronic disease management to vaccine delivery and gene therapy, and they present unprecedented opportunities for modern medicine. Through refined carrier design, precise drug packaging, innovative targeting strategies, and comprehensive characterization evaluation, BOC Sciences is committed to developing more efficient and safer drug delivery systems that benefit patients and drive the development of biomedical sciences to new heights.

FAQ

1. What types of proteins are used in your nanoparticle drug delivery systems?

We utilize a variety of proteins, including albumin, gelatin, and silk fibroin, each chosen based on their biocompatibility, biodegradability, and specific properties that make them suitable for different therapeutic applications.

2. How do you ensure the stability of protein-based nanoparticles?

Stability is ensured through rigorous testing under various conditions to evaluate the integrity and functionality of the nanoparticles over time. We optimize formulation parameters and employ advanced stabilization techniques to enhance the shelf-life and robustness of the nanoparticles.

3. Can you customize nanoparticles for specific therapeutic targets?

Yes, we can functionalize protein-based nanoparticles with specific ligands, antibodies, or peptides to target particular cells or tissues. This customization enhances the therapeutic efficacy and reduces off-target effects, providing a more precise treatment.

4. What therapeutic areas can benefit from protein-based nanoparticle drug delivery systems?

These systems are versatile and can be used in various therapeutic areas, including cancer therapy, chronic disease management (e.g., diabetes, rheumatoid arthritis), and regenerative medicine. They offer targeted delivery, controlled release, and enhanced stability for a wide range of treatments.

5. Can you scale up production for clinical trials and commercial use?

Absolutely. We focus on developing scalable processes that can be translated from laboratory-scale to large-scale production without compromising quality. This ensures that the protein-based nanoparticles are suitable for clinical trials and commercial use.

6. What sets your protein-based nanoparticle drug delivery systems apart from other drug delivery methods?

Our systems offer several advantages, including biocompatibility, biodegradability, targeted delivery, controlled release, and enhanced stability. These properties make them superior to traditional drug delivery methods, leading to improved therapeutic outcomes and reduced side effects.