Hyaluronic Acid Modification
BOC Sciences offers top-grade hyaluronic acid (HA) modification solutions to fulfill industry needs for HA derivatives. Hyaluronic acid functions as a natural polysaccharide and finds applications in pharmaceuticals, cosmetics, and food production. The physicochemical properties of HA can experience significant changes through chemical or biological modifications, which improve its functional performance across various applications. Our dedication lies in utilizing advanced technologies to modify HA through various approaches so we can deliver custom solutions tailored to evolving market needs. BOC Sciences delivers outstanding products and services featuring crosslinked HA, non-crosslinked HA, and biological and surface modifications to secure superior quality and reliability for research and commercialization support.
What is Hyaluronic Acid Modification?
HA modification refers to altering the structure of HA through chemical or physical means to enhance its functionality for specific applications. HA itself possesses good biocompatibility, moisturizing properties, and viscoelasticity, but in many applications, pure HA may not meet the requirements. By methods such as crosslinking, substitution, or surface modification, properties such as molecular weight, solubility, stability, and degradability can be adjusted to better suit diverse applications like drug delivery, tissue engineering, and skincare. For example, in drug delivery systems, crosslinked HA can improve stability and control drug release, while non-crosslinked HA is often used in moisturizing and lubricating products. Modified HA not only enhances its original advantages but also imparts new functions, giving it broader applications in biomedicine, cosmetics, and other fields.
Technical Advantages of BOC Sciences in Hyaluronic Acid Modification
Advanced Modification Technology Platform
We offer tailored solutions with chemical, physical, surface, and biological modification technologies for diverse needs.
Professional Research Team
Our team of chemists and biologists provides expert support in HA modification with interdisciplinary experience.
Customized Modification Solutions
We offer flexible technical support to adjust molecular weight, improve solubility, and impart specific biological activities.
Strict Quality Control System
BOC Sciences ensures stability and reliability of each modified HA batch through strict quality management.
Efficient Production Capacity
We adjust production scales to meet varying business needs, ensuring timely delivery for both small and large orders.
Cutting-Edge Analytical Capabilities
Advanced tools enable precise HA characterization and optimization, meeting client-specific quality standards.
Crosslinked Hyaluronic Acid Modification Services
HA crosslinking refers to the reaction between HA and crosslinking agents that contain relevant functional groups, resulting in a molecular network structure with varying degrees of crosslinking. This process leads to the growth of HA molecular chains, an increase in average molecular weight, enhanced viscoelasticity, relatively weakened water solubility, and improved mechanical strength. In our crosslinked HA modification services, BOC Sciences offers customized crosslinking strategies to meet different drug delivery needs while controlling the crosslinking degree and molecular weight to ensure optimal performance.
Hydrazide Crosslinking
Hydrazide compounds act as crosslinking agents, transforming flowable gels into brittle gels with greater mechanical hardness. The most commonly used crosslinking agent is adipic acid dihydrazide (ADH), and when present in large amounts, it allows the formation of stable HA-ADH derivatives.
Sulfonate Crosslinking
At room temperature, divinyl sulfone (DVS) rapidly crosslinks with the hydroxyl groups of HA to form gels with varying properties. By controlling the concentration of HA, molecular weight, HA/DVS ratio, and the pH of the reaction medium, the degree of gel crosslinking can be adjusted.
Carbodiimide (EDC) Crosslinking
Carbodiimide (EDC) reacts with the carboxyl groups of HA in acidic solutions, forming N-acylurea compounds that then react with different carbodiimides, resulting in stable, rigid, bio-dense, and resistant crosslinked derivatives with high resistance to hyaluronidase degradation.
Photocrosslinking
Photocrosslinked HA undergoes a chemical reaction initiated by ultraviolet light, promoting intermolecular crosslinking to form a three-dimensional network structure. This method is simple and controllable, suitable for preparing hydrogel materials with good biocompatibility for biomedical applications.
BDDE Crosslinking
1,4-Butanediol diglycidyl ether (BDDE) crosslinks HA by reacting the ether groups of BDDE with the hydroxyl groups in HA molecules, forming a stable crosslinked structure. This crosslinking method is widely used in preparing hydrogels and biomaterials.
Polyethylene Glycol Crosslinking
Polyethylene glycol (PEG) crosslinks HA through reactions between PEG and the carboxyl or hydroxyl groups in HA, forming a controllable crosslinked network. This crosslinking enhances material stability and improves biocompatibility and mechanical properties.
DVS Crosslinking
Divinyl sulfone (DVS) crosslinks HA by reacting the bifunctional vinyl groups of DVS with the carboxyl groups in HA, forming a crosslinked structure. This method is commonly used to enhance the mechanical strength and thermal stability of HA hydrogels.
Bispoxirane Crosslinking
Bispoxirane crosslinks HA by reacting its two epoxy groups with the hydroxyl or carboxyl groups in HA, forming a crosslinked network structure. This crosslinking method is efficient and can be used to enhance the stability and biocompatibility of HA-based materials.
Non-Crosslinked Hyaluronic Acid Modification Services
Non-crosslinked HA modification aims to improve its physicochemical properties, such as solubility, moisturizing ability, and stability. Non-crosslinked HA is widely used in cosmetics, ophthalmic treatments, joint lubrication, and skincare. In this service, BOC Sciences modifies the HA molecules using methods such as chemical modification, polymerization, or esterification to meet the specific needs of various industries for HA functionality. Our technology precisely controls the molecular weight and properties of its derivatives, ensuring optimal performance in end-use applications.
Esterification Modification
Esterification involves the reaction of the hydroxyl group in HA with acids or acid anhydrides, or the reaction of the carboxyl group with alcohols, phenols, or epoxides, forming ester derivatives of HA. This improves its hydrophobicity or adjusts its solubility.
Grafting Modification
The grafting reaction of HA involves attaching small molecules or polymers to the main chain of HA. Researchers have grafted HA with high-density polyethylene (HDPE) to create a biomaterial suitable for bone tissue repair.
Hydrophobic Modification
Modifying sodium hyaluronate with hexadecyltrimethylammonium bromide creates hydrophobic CTA-HA, while grafting end-capped polylactic acid forms the degradable CTA-HAOLA, which self-assembles into a hydrogel in aqueous solutions.
Amination Modification
The introduction of amino groups modifies the structure of HA molecules, enhancing their interaction with proteins or cells. This is often used to improve bioactivity or enhance the performance of drug carriers.
Etherification Modification
Etherification involves the reaction of the hydroxyl group in HA with alcohols, forming ether bonds, thereby altering its solubility, hydrophilicity, and biocompatibility.
Methylation Modification
Methylation of the hydroxyl groups of HA with methylating agents results in methylated HA, typically used to improve the stability of HA and reduce its degradation rate.
Sulfonation Modification
Sulfonating HA introduces sulfonic acid groups into the molecule, altering its physicochemical properties by increasing water solubility and anticoagulant properties.
Acetylation Modification
Acetylation introduces acetyl groups to improve the stability and solubility of HA, commonly used in the preparation of biomedical materials or drug carriers.
Surface Modification Services for Hyaluronic Acid Supported by BOC Sciences
BOC Sciences offers HA surface modification services, utilizing advanced surface engineering techniques to improve the performance of HA in specific applications, particularly in biomedicine, drug delivery, cosmetics, and materials science. By chemically modifying, physically modifying, or composite material modifying the surface of HA, BOC Sciences can provide HA derivatives with enhanced biocompatibility, stability, and targeting capabilities. Through precise modification methods, we enhance HA's performance in various applications, meeting diverse client needs in research and production. We support the modification of the following molecules:
Enzymes Collagen Nucleic Acids Peptides Liposomes Polymers Nanomaterials Metal Ions
Dialysis and Washing Services for Hyaluronic Acid Gel Beads
In the production of HA gel beads, dialysis and washing techniques play a critical role in ensuring the removal of residual harmful substances, such as endotoxins, foreign materials, and crosslinking agents that may remain after the crosslinking process. The core purpose of dialysis and washing is to improve the safety and quality of the final product, ensuring compliance with FDA requirements for endotoxins and residual crosslinking agents. The HA gel bead dialysis and washing services provided by BOC Sciences strictly follow industry standards and regulatory requirements, ensuring that the products meet the FDA-approved endotoxin and residual crosslinking agent thresholds. Our dialysis and washing process is optimized to effectively remove harmful substances while maximizing the protection of HA gel bead quality and stability. We are committed to providing high-quality, safe, and reliable HA gel beads to meet the needs of customers worldwide.
Comprehensive Modification Analysis and Quality Control System
BOC Sciences offers a comprehensive HA modification analysis and quality control service, ensuring that each batch of modified HA meets rigorous standards throughout product development and application processes. As an essential biopolymer, HA's applications in biomedicine, drug delivery, and tissue engineering require not only high purity but also the assurance of biocompatibility, stability, and functionality. To meet these demands, BOC Sciences employs advanced analytical technologies and strict quality control procedures to ensure that the performance characteristics of modified HA align with industry standards and meet specific customer requirements.
Detection Platforms
Nuclear Magnetic Resonance (NMR) Fourier-Transform Infrared Spectroscopy (FTIR) Ultraviolet-Visible Spectroscopy (UV-Vis) High-Performance Liquid Chromatography (HPLC) Gel Permeation Chromatography (GPC) Mass Spectrometry (MS) Gas Chromatography (GC) Liquid Chromatography-Mass Spectrometry (LC-MS) Thermogravimetric Analysis Cell Culture Experiments
Analysis Projects
Molecular Structure Characterization Crosslinking Degree Analysis Purity Testing Impurity Detection Biocompatibility Testing Cytotoxicity Testing Hydration Analysis Viscosity Measurement Stability Testing Storage Condition Testing
Service Workflow
Research and Production Applications of Modified Hyaluronic Acid
Modified HA, especially crosslinked HA, demonstrates immense potential in various research and production applications due to its unique physicochemical properties. Crosslinked HA enhances stability, viscosity, and hydration through physical or chemical crosslinking, making it applicable in biomedicine, cosmetics, food, drug delivery, and tissue engineering. Its use in crosslinked HA gels presents unparalleled advantages in applications such as drug delivery systems, tissue repair materials, and biosensors.
Cosmetics
Crosslinked HA is widely used in cosmetics, particularly in anti-aging and moisturizing products. Its gel characteristics help lock in moisture, enhancing skin elasticity and smoothness.
Medical Fillers
Crosslinked HA gels are commonly used in medical fillers, particularly in plastic and reconstructive surgery. They can fill wrinkles and enhance facial contour fullness.
Drug Delivery
Crosslinked HA enables slow drug release in the body, prolonging therapeutic effects. It is extensively used in the delivery systems for anticancer and anti-inflammatory drugs.
Tissue Engineering
In tissue engineering, crosslinked HA gels serve as cell carriers and scaffold materials, promoting cell adhesion and growth by providing a conducive environment for new tissue formation.
Wound Healing
Crosslinked HA gels are widely used in wound healing, offering a moist environment that promotes epithelial cell growth and healing.
Biosensors
Crosslinked HA gels serve as carriers in biosensors, enhancing the sensor's stability and response speed.
Food Industry
Crosslinked HA is used as a thickener and stabilizer in the food industry. It improves the texture, taste, and shelf life of food products.
Ophthalmology
Crosslinked HA is used in ophthalmic surgeries as a surgical gel or lubricant, particularly in eye surgeries or treatments for dry eyes.
FAQs
What is crosslinked hyaluronic acid?
Crosslinked hyaluronic acid is hyaluronic acid where molecules are chemically linked to form a three-dimensional network structure. After crosslinking, the molecular structure of hyaluronic acid becomes more stable, enhancing its persistence and efficacy in the skin or tissue. It is commonly used in injectable fillers, skincare, and joint treatments.
What is non cross linked hyaluronic acid?
Non-crosslinked hyaluronic acid refers to hyaluronic acid that has not undergone the crosslinking process. Its molecular structure is more loosely bound and is typically used in lubrication, moisturizing, and basic skincare products. Because it lacks crosslinking, non-crosslinked hyaluronic acid degrades faster in the body, offering shorter-lasting effects.
How is hyaluronic acid crosslinked?
Crosslinking agents for hyaluronic acid typically include chemical reagents such as 1,4-butanediol diglycidyl ether (BDDE), glutaraldehyde, isocyanates, and thiocyanates. These agents form chemical bonds, such as covalent bonds or disulfide bonds, to enhance the stability and longevity of hyaluronic acid.
What are the crosslinking agents for hyaluronic acid?
Crosslinking agents for hyaluronic acid commonly include chemical reagents like 1,4-butanediol diglycidyl ether (BDDE). These agents form chemical bonds between hyaluronic acid molecules, stabilizing the structure and enhancing its effects in the skin or joints.
Is cross-linked hyaluronic acid safe?
Crosslinked hyaluronic acid is generally safe after undergoing rigorous quality control and clinical testing. It is widely used in medical and cosmetic fields, such as skin fillers and joint treatments. However, individuals may have allergic reactions or side effects, so it is important to follow medical advice when using it.