When you work with BOC Sciences, you can leverage our state-of-the-art facilities and experience and expertise in tissue distribution studies and quantitative whole-body autoradiography (QWBA) to evaluate your drug candidates to understand the relationship between the distribution and accumulation of your compounds and/or their metabolites, to reveal tissue-specific activity, and to identify potential toxicity. Which method is right for you?
Drug tissue distribution is a complex process that relies on a long list of parameters that affect the delivery, retention, and removal of drugs into and out of biological tissue.
Single-dose tissue distribution studies provide information for designing toxicology and pharmacology experiments and for interpreting the results of these studies.
Repeat dose tissue distribution studies are designed for compounds with longer half-lives in tissue compared to plasma, drugs for site-specific targeted delivery systems, incomplete elimination or unanticipated organ toxicity.
Tissue distribution studies play a vital role in drug development programs:
Traditionally, investigations have been carried out by conventional methods, such as tissue dissection, total radioactivity determination, and LC-MS-MS/QPCR. However, the advent of quantitative whole-body autoradiography (QWBA) has revolutionized drug distribution studies by utilizing fluorescence imaging techniques and providing high resolution images.
QWBA is a powerful tool for studying the systemic distribution of subjects and the elimination patterns of radiolabelled drugs in various tissues. Its advantages include:
QWBA can be performed alone or in combination with radiolabeled mass balance (MB) and plasma pharmacokinetic (PK) studies to provide high-resolution quantitative data candidates that can be used to characterize drug absorption, distribution, metabolism and excretion (ADME) properties.
Fig. 1 Autoradiograms of selected sections of mice at 48 h after dosing with [3H]BMS-753493 (Shen H, 2016)
To initiate a QWBA study, our researchers administered radiolabeled compounds to animals of interest. At a specific time point after administration (usually within 168 hours), the animals are humanely euthanized and the carcasses are frozen. The carcass is then prepared in a solid block embedded in a medium. Next, thin sections of the entire animal carcass are created using a cryostat and these sections are analyzed to determine where the compound is distributed in the body.
|Route of Administration
|Mouse (including chimeric with humanized liver)
|Animal models of human disease (knockout models)
As with all of our live services, we make sure to keep in touch during the study. If you have any special needs or questions about our services, please feel free to contact us to support our experienced experts. We look forward to working with you in the future.