BOC Sciences' high-throughput bioscience laboratory is staffed by qualified members with advanced training in biology, chemistry, and engineering. We have extensive experience in target identification and validation, assay development, high-throughput screening, biochemical assays, and biophysical analysis. We assist our clients with assay development, analytical validation, evaluation and development of new drug discovery technologies.
High throughput refers to the analysis of those large numbers of sequences using computers and related machines that can perform multiple samples at a time.
The first generation sequencing can only test a segment of sequence of one sample, which generates relatively small amount of data. In contrast, high-throughput sequencing can generate more than hundreds of data each time, and with computer analysis to process the corresponding test data, more samples can be tested each time, and more information can be obtained from it, so that valuable information can be screened in it.
High-throughput biology and screening help us to discover innovative drugs. The quality, novelty and development potential of these chemical entities drive the success of your drug discovery program.
Biochemical assays and cell-based assays are the two main categories of drug discovery. Biochemical assays are useful for evaluating the interaction between compounds and targets/target proteins, which is consistent and simple. Cell-based assays help identify the target expression at the cellular level and provide a functional read-out of compound activity.
A biochemical assay is an in-vitro procedure used to detect, quantify and study the binding or activity of biomolecules. Biochemical assay development and analysis are used as routine and reliable procedure to aid target characterization and enable a valuable understanding of biomolecular functions during drug discovery and development.
Biophysical assays enable an understanding of drug-target interactions at the molecular level. Primary and secondary biophysical screening selection is performed to determine the residence time, mechanism of action, and kinetics of the drug, all of which are critical for the therapeutic effect of the drug. Therefore, biophysical screening is essential for drug discovery.
Cellular assays (or Cell-based assays) are becoming the preferred tool for screening potential drug compounds. The use of cellular assays for lead identification and optimization has become a growing trend in drug discovery because they can provide more relevant physiological information than biochemical assays. In contrast to assays based on biochemical targets, cellular assays typically do not assume priori knowledge of direct molecular targets. In contrast, many cellular assays aim to identify modulators of pathways of interest in the cellular physiological environment, complete regulatory networks and feedback control mechanisms.
High-throughput screening is based on experimental approaches at the molecular and cellular levels. It takes the microplate as the experimental tool carrier, then performs the test process with an automated operating system. Experimental data is automatically collected with sensitive and fast detection instruments. HTS can test tens of millions of samples at the same time and get the corresponding database to support the operation of the technical system. HTS technology provides more rapid, sensitive, and accurate tools compared with conventional method.
Protein production is a biotechnological process that produces a specific protein. It usually involves manipulation of gene expression in an organism. Expression involves transcription of recombinant DNA into messenger RNA (mRNA), translation of mRNA into polypeptide chains, and ultimately folding into functional proteins that can be targeted to specific subcellular or extracellular locations.