Peptide In Vitro and In Vivo Testing
BOC Sciences has an advanced platform for peptide research, preparation, and evaluation, capable of providing comprehensive testing services for peptide raw materials, peptide microspheres, peptide nanoparticles, and peptide formulations. These services cover both cellular and animal-level testing. In vitro testing is supported by a high-standard cell platform, enabling efficacy validation and safety analysis for various functions such as anti-aging, whitening, anti-inflammatory, and antibacterial evaluations. For in vivo testing, we leverage our expertise in animal model development to conduct systematic studies on the pharmacodynamics, toxicology, skin permeability, and tissue distribution of peptides. Our platform is fully equipped, with standardized data output and highly flexible customization capabilities, meeting the research and regulatory needs of clients from industries such as medical aesthetics, cosmetics, and biomedicine at different stages of development.
Challenges in Peptide Analysis? BOC Sciences is Here to Help!
Lack of Systematic Methods for Efficacy Validation
Many functional peptides (such as anti-wrinkle, whitening, and repair peptides) are difficult to evaluate accurately using traditional cell models. BOC Sciences provides various cell models (e.g., human dermal fibroblasts, keratinocytes, melanocytes) and standardized efficacy evaluation metrics, enabling quantitative verification of peptide functions.
Difficulty in Characterizing Delivery Systems In Vitro
The behavior of peptide microspheres, nanoparticles, and other sustained-release carriers in cell models is complex and difficult to characterize using conventional methods. We use techniques like fluorescence tracking, dynamic particle size monitoring, and confocal microscopy to systematically study the release and cellular uptake mechanisms of these systems in vitro.
Challenges in Permeability and In Vivo Distribution Assessment
The permeability of peptide formulations through the skin and their distribution within the body directly impact their efficacy. BOC Sciences has established skin permeability models and small animal imaging technology platforms, allowing for dynamic analysis of peptide distribution in skin and targeted tissues.
High Barriers in Animal Model Development
Animal model construction for skin aging, whitening, inflammation, and other areas is challenging. BOC Sciences has an experienced animal research team skilled in developing models for photoaging, melanin deposition, wound healing, and more, meeting the preclinical efficacy validation requirements.
Insufficient Toxicology and Irritation Assessment
Different types of peptides and their carrier systems may cause cytotoxicity or skin irritation. We offer multidimensional safety evaluations, including acute toxicity testing, apoptosis analysis, and skin sensitization assays, ensuring that products meet regulatory or market compliance standards.
Lack of Systematic Experimental Design for Regulatory Submission
Many clients lack a systematic approach during the experimental design phase, making it difficult to convert data into submission materials. BOC Sciences has an interdisciplinary R&D team to assist clients from project initiation and design through experiment execution and data archiving, ensuring results are scientific, compliant, and suitable for filing or registration.
Peptide In Vitro Testing Services Based on Cell Platforms
BOC Sciences possesses an advanced cell testing platform focused on evaluating peptide products' efficacy and safety at the cellular level, supporting comprehensive testing from basic mechanism studies to data required for product registration. With a mature testing system, we can accurately assess peptide functions like anti-aging, whitening, moisturizing, anti-inflammatory, repair, and antioxidant effects. Additionally, we are equipped with a professional research team and automated analysis equipment, capable of high-throughput screening, imaging analysis, biomarker detection, and more. We can customize experimental plans to meet clients' needs and provide standardized, traceable, and registration-ready data reports. These services are widely applied in the development of peptide products in the medical aesthetics, cosmetics, and biopharmaceutical industries.
Peptide Bioactivity Evaluation
- Antioxidant Activity Testing: DPPH, ROS, SOD, CAT level assays.
- Anti-inflammatory Activity Testing: NO release, IL-6/TNF-α/IL-1β expression.
- Anti-aging Analysis: MMPs expression detection, SA-β-gal staining.
- Whitening Efficacy Validation: Tyrosinase activity inhibition, melanin content measurement.
- Antibacterial Testing: Evaluation of inhibition against common skin bacteria (e.g., Staphylococcus aureus, Propionibacterium acnes).
Safety and Toxicity Analysis
- Cell Viability Testing: MTT, CCK-8, LDH release assays.
- Cytotoxicity Assessment: Flow cytometry for apoptosis/necrosis detection.
- Skin Irritation Prediction Model: Reconstructed human epidermis model/keratinocyte response.
Peptide Sustained-release System Behavior Study
- Particle Uptake and Localization: FITC-labeled tracking of nanoparticles within cells.
- Release Kinetics Study: Evaluation of microsphere/nanoparticle release characteristics in vitro.
- Impact of Carriers on Cells: Particle size, surface charge effects on cell viability.
Peptide In Vivo Testing Services Based on Animal Platforms
With extensive experience in working with mice, rats, and other experimental animals, we can develop various animal models that meet application-specific needs, including skin aging, melanin deposition, wound healing, inflammation response, and tissue regeneration. These models are particularly suited for peptide function verification and mechanism research in medical aesthetics and biopharmaceutical fields. We have systematic procedures for experimental design, dose assessment, administration routes (e.g., subcutaneous injection, topical application, oral administration), sample collection, and tissue analysis, supporting pharmacokinetics (PK), pharmacodynamics (PD), toxicology, and skin permeability studies. This ensures that our results are highly scientific, reproducible, and suitable for regulatory submission, helping clients efficiently advance peptide products through preclinical development.
Peptide Pharmacodynamics Evaluation
- Anti-wrinkle and Firming Testing: Establish UV-induced photoaging models, detect collagen and elastin changes.
- Anti-inflammatory and Repair Ability: Use mouse ear inflammation and stratum corneum damage models for efficacy assessment.
- Whitening Efficacy Verification: Melanin deposition models, mouse skin pigmentation inhibition analysis.
- Antibacterial and Immune Modulation: Inflammation factor regulation and immune cell analysis in bacterial infection models.
Animal Tissue Pathological Analysis
- HE Staining: Tissue morphology and structural changes assessment.
- Masson Staining: Collagen formation assessment.
- Immunohistochemistry and Immunofluorescence: Key protein expression analysis (e.g., MMP-1, TGF-β, IL-6).
Peptide Release and Distribution Study
- Biodistribution: Fluorescent/radiolabeling analysis of peptide accumulation in skin or organs.
- In Vivo Release Curve Analysis: Pharmacokinetic modeling of sustained-release peptide carriers.
- Skin Penetration Study: Analysis of penetration depth via transdermal administration models.
Safety Assessment
- Skin Irritation and Sensitization Tests: Draize test, ear swelling test.
- Acute/Subchronic Toxicity Testing: Observation of behavioral, physiological, and organ tissue changes after peptide administration.
Comprehensive Cell and Animal Platforms for Peptide Evaluation
BOC Sciences boasts a comprehensive cell and animal experimental infrastructure, establishing a multi-dimensional analytical system ranging from molecular to tissue, organ, and whole physiological systems. The cell platform includes keratinocytes, human dermal fibroblasts, melanocytes, skin microbiome models, and more, supported by precise instruments such as flow cytometers, confocal microscopes, and quantitative PCR. The animal platform is equipped with AAALAC-certified SPF-grade animal housing and supports the establishment of various skin models and pharmacological evaluations. It is further complemented by a professional team for animal behavior monitoring and tissue pathology analysis. With the synergistic operation of these two platforms, BOC Sciences provides comprehensive in vitro and in vivo evaluation pathways for peptide products.
Cell Platforms
Fibroblast Cell Lines Keratinocyte Cell Lines Endothelial Cell Lines Immune Cell Lines Nerve Cell Lines Adipocyte Cell Lines
Animal Platforms
Mouse Models Rat Models Rabbit Models Pig Models Dog Models Monkey Models
Applications of Peptides in Medical Aesthetics and Cosmetics
Peptides, as highly active functional ingredients, are widely used in medical aesthetics and cosmetics due to their excellent biocompatibility, targeting abilities, and diverse biological functions. Whether raw peptides or peptide formulations based on nanoparticle, microsphere, or gel delivery systems, these can achieve superior permeability, stability, and prolonged efficacy. BOC Sciences is committed to in vitro and in vivo testing services for peptide products, evaluating anti-aging, whitening, repair, anti-inflammatory, and other functional effects using skin-related cell models and animal models. This provides robust data support and R&D guarantees for peptide-based skincare products, injectable products, and topical formulations, facilitating the progression of peptides from R&D to clinical and consumer markets.
Dermal Fillers
The use of peptides in dermal fillers helps restore skin elasticity and firmness. By promoting collagen and elastin fiber production, these fillers effectively improve skin sagging or wrinkles caused by aging or facial defects. Peptide components can enhance the effectiveness of the filler, improve the dermal layer structure, and with long-term use, help maintain the natural uplift of facial contours, achieving smoother and fuller results.
Anti-Aging Serums
Peptides are widely used in anti-aging serums, mainly by stimulating collagen and elastin synthesis to improve skin structure. These products typically contain peptides that promote cell renewal and have antioxidant properties, effectively reducing fine lines and wrinkles, leaving the skin firmer and smoother.
Whitening Creams
Peptides are often added to whitening creams to inhibit melanin production and reduce dark spots and uneven skin tone. Peptides regulate skin pigment metabolism, improving skin tone to make it brighter and more even, catering to consumers with skin whitening needs.
Repair Masks
Peptides are commonly used in repair masks to promote skin repair and regeneration. Peptides accelerate skin cell regeneration, repairing damage caused by UV rays, environmental pollution, or other external stimuli, helping alleviate skin dryness, redness, or inflammatory reactions.
Moisturizing Lotions
Peptides are widely applied in moisturizing lotions to help maintain the skin's moisture balance. These products strengthen the skin barrier function, reduce water loss, and promote the synthesis of natural moisturizing factors, keeping the skin hydrated for a longer time.
Vaccine Development
Peptides are widely used in vaccine development, especially in synthetic vaccines. By synthesizing specific pathogen antigen peptides, they can stimulate immune responses to help prevent various infectious diseases. The advantages of peptide vaccines include high efficiency, low cost, and good stability.
Targeted Drug Delivery
Peptides, as part of targeted drug delivery systems, can enable precise drug delivery by binding to specific receptors on cell surfaces. By modifying peptides, the stability of drugs in the body and their selectivity for target cells can be improved, enhancing therapeutic effects and reducing side effects.
Cancer Treatment
Peptides play a significant role in cancer treatment. By designing peptides with specific targeting properties that inhibit tumor cell growth, they can directly affect tumor cells, suppressing their proliferation or promoting apoptosis. Additionally, some peptides can be used in cancer immunotherapy, enhancing the immune system's ability to recognize and attack tumor cells.
FAQs
What is the lab test for peptides?
The lab test for peptides typically involves techniques such as High-Performance Liquid Chromatography (HPLC) to assess purity and identity, Mass Spectrometry (MS) for molecular weight determination, and UV-Vis spectrophotometry for concentration measurement. These tests help confirm the peptide's structure, composition, and quality by evaluating factors such as molecular integrity, purity, and the presence of impurities.
What is the identification test for peptide?
The identification test for peptides often includes mass spectrometry (MS) and amino acid analysis. Mass spectrometry helps determine the molecular weight and sequence of the peptide, confirming its identity. Additionally, Edman degradation or liquid chromatography-tandem mass spectrometry (LC-MS/MS) can be used to sequence peptides and verify their structure, offering precise identification by comparing it with known peptide databases.
Can you drug test for peptides?
Yes, drug testing for peptides is possible using methods like liquid chromatography-mass spectrometry (LC-MS/MS), which can detect specific peptide sequences in biological samples such as blood or urine. Peptides used in performance enhancement or illicit drug use can be identified, quantitated, and monitored for any abnormal levels, enabling their detection during anti-doping tests or clinical drug screenings.