Hyaluronic Acid Preparation
Both hyaluronic acid and its sodium salt, sodium hyaluronate, serve as versatile biopolymers for use in the pharmaceutical industry as well as cosmetic and food sectors. BOC Sciences offers hyaluronic acid preparation and production services at both laboratory-scale and industrial-scale levels due to their extensive biotechnology research and development background. Through the use of advanced extraction and fermentation technologies and strict quality control systems, our processes deliver high purity and stable products that maintain consistent quality across pharmaceutical, cosmetic, and food industry standards.
What is Hyaluronic Acid?
Hyaluronic acid (HA) exists naturally as a high-molecular-weight polysaccharide within human and animal tissues and is a member of the glycosaminoglycan (GAG) family. The widespread use of this substance in skincare products and medical applications such as orthopedic fillers and ophthalmic surgery stems from its superior ability to retain water alongside its lubricating properties and biocompatibility. HA production currently relies primarily on two methods, which are animal tissue extraction and microbial fermentation. The original production method for HA extraction involved obtaining the substance from animal tissues like rooster combs and bovine vitreous humor. Despite its established status, the animal tissue extraction method faces challenges stemming from limited raw material availability combined with inefficient extraction processes and possible pathogen contamination risks. Genetically engineered microorganisms like Streptococcus serve as the production basis for microbial fermentation. The production method stands out as mainstream because it provides abundant raw material sources alongside high production efficiency and product purity. Additionally, research is ongoing on chemical synthesis and enzymatic methods, but these techniques have not yet reached widespread industrial application.
Technical Advantages of BOC Sciences in Hyaluronic Acid Preparation
Advanced Fermentation Processes
Optimization of strain selection and culture conditions to enhance HA yield and control molecular weight.
Strict Quality Control
GMP-grade production systems ensure product purity, molecular weight uniformity, and biological safety.
Diverse Product Specifications
Custom production of HA ranging from low molecular weight ( < 10 kDa) to high molecular weight ( > 1000 kDa).
Advanced Purification Technologies
Use of ultrafiltration, chromatography, ion exchange, and high-performance liquid chromatography to ensure high purity and activity.
Comprehensive Application Support
Providing HA products that meet the standards of pharmaceutical, cosmetic, food, and biomedical industries.
Customized Services
Offering tailored solutions for different molecular weights, structural modifications, and functionalized HA based on customer needs.
High Purity Hyaluronic Acid Reliable for Pharmaceutical and Cosmetic Use
BOC Sciences supports the preparation of various types of HA, including low molecular weight HA (for anti-inflammatory and wound healing applications), medium molecular weight HA (for moisturizing in cosmetics), and high molecular weight HA (for joint lubrication and ophthalmic surgery). Additionally, we offer a wide range of products such as sodium hyaluronate, crosslinked HA, and HA derivatives to meet the needs of different applications.
High Molecular Weight HA
With a molecular weight exceeding 2000 KDa, it possesses excellent viscoelasticity, lubricity, and moisturizing properties. In cosmetics, it is mainly used as a moisturizer, forming a protective film on the skin surface without being absorbed by the skin.
Medium Molecular Weight HA
With a molecular weight ranging from 500 KDa to 2000 KDa, it has diverse applications in cosmetics, pharmaceuticals, and health supplements. In cosmetics, its primary function is moisturizing, forming a breathable film on the skin’s surface to lock in moisture and prevent evaporation, ensuring long-lasting hydration.
Low Molecular Weight HA
With a molecular weight between 10 KDa and 500 KDa, it is easily absorbed by the skin and participates in skin metabolism. It has certain effects in anti-inflammation, wound healing, and anti-aging, though its ability to prevent moisture evaporation is relatively weak.
Oligomeric HA
With a molecular weight of less than 10 KDa, containing fewer than 50 monosaccharide units and a polymerization degree of less than 25, this type of HA can penetrate the dermis, inhibit the release of inflammatory factors, alleviate sensitivity caused by various factors, and repair epidermal and fibroblast damage.
Sodium Hyaluronate
The sodium salt form of HA offers greater stability and stronger permeability while retaining similar efficacy to HA.
Acetylated HA
Produced through acetylation, the introduction of acetyl groups enhances the lipophilicity of HA, improving its affinity and adhesion to the skin. It provides stronger moisturizing effects than regular HA.
Fluorescent-Labeled HA
Used in bioimaging and cell-tracking research, HA molecules conjugated with fluorescent markers enable the visualization of cellular absorption and metabolism processes.
Crosslinked HA Polymer
An ultra-high molecular weight polymer obtained through crosslinking reactions, varying in crosslinking degree and molecular weight. This type of HA polymer can be used as injectable fillers for breast augmentation and wrinkle reduction.
Advanced Hyaluronic Acid Preparation Technologies to Meet Diverse Customer Needs
BOC Sciences provides various advanced HA preparation technologies to ensure that different customer requirements and industry applications are met. Our HA production technologies primarily include animal tissue extraction and microbial fermentation, each with its unique advantages, allowing customized solutions tailored to different applications and product specifications.
Animal Tissue Extraction
BOC Sciences possesses advanced expertise in this field, enabling efficient HA extraction using enzymatic hydrolysis, separation, and purification techniques. These methods maximize the retention of HA's bioactivity and molecular weight characteristics during the extraction process.
Microbial Fermentation
BOC Sciences utilizes high-yield HA-producing microbial strains (such as Streptococcus, Lactobacillus, and Escherichia coli). By optimizing culture media formulations, cultivation conditions, and fermentation process controls, we ensure high production efficiency, precise molecular weight distribution, and reduced production costs while maintaining batch-to-batch consistency.
Customized Preparation
BOC Sciences also offers a variety of customized HA preparation technologies. We can adjust molecular weight, crosslinking degree, and sulfation levels to meet specific functional, structural, and performance requirements. Additionally, we can develop HA derivatives with specialized functionalities.
GMP Manufacturing Capacity from Laboratory to Industrial Scale
BOC Sciences has robust large-scale manufacturing capabilities in HA (sodium hyaluronate) production, adhering strictly to GMP certification to ensure high quality and safety at all stages. We provide HA production from laboratory-scale to industrial-scale, covering gram, kilogram, and ton-level supplies to flexibly meet customer demands. As a GMP-certified manufacturer, BOC Sciences strictly follows international quality management standards throughout the production process, from raw material procurement, manufacturing processes, and quality testing to product packaging. Equipped with world-class facilities and advanced production equipment, we maintain highly standardized production workflows to ensure that each batch of products meets pharmaceutical, cosmetic, and food-grade quality standards.
Hyaluronic Acid Modification Services from BOC Sciences
BOC Sciences provides professional HA modification services, aiming to optimize the performance of HA through various chemical modification methods to meet customers' specific needs in different application fields. We employ a range of advanced modification techniques, including esterification, crosslinking, and graft copolymerization, enabling the introduction of different functional groups or enhancement of specific properties within the HA molecular structure. These modification methods allow for molecular weight adjustment, improved solubility, enhanced stability, and increased biocompatibility of HA. In crosslinking modification, BOC Sciences utilizes crosslinking agents such as glutaraldehyde and diisocyanates, employing chemical crosslinking technology to reinforce the three-dimensional network structure of HA, thereby improving its viscosity and stability in aqueous solutions. Crosslinked HA is widely used in the biomedical field, including drug delivery systems and tissue engineering scaffolds, helping to enhance drug release properties, improve tissue repair capabilities, and extend product lifespan.
Stringent Hyaluronic Acid Purification and Quality Testing Services
To ensure high purity and quality, BOC Sciences offers efficient purification solutions, including ultrafiltration, ion exchange, gel filtration, and membrane separation technologies. Our purification processes effectively remove impurities, endotoxins, and other contaminants, enhancing the biocompatibility and safety of the final product. Whether for pharmaceutical, cosmetic, or food applications, our purification services meet rigorous industry standards to ensure premium product quality. In addition, BOC Sciences employs a stringent analytical testing and quality control system to ensure that our HA products comply with international standards.
Advanced Analytical Platform
Ion Exchange Chromatography (IEC) Gel Permeation Chromatography (GPC) Size Exclusion Chromatography High-Performance Liquid Chromatography (HPLC) Reverse Phase HPLC (RP-HPLC) Gas Chromatography (GC) Ultraviolet-Visible Spectrophotometry (UV-Vis)
Quality Testing Services
Molecular Weight Testing Purity Analysis Viscosity Testing Structural Characterization Endotoxin Testing Microbial Limit Testing Physicochemical Property Analysis Biocompatibility Evaluation
Service Workflow
What Applications Do Hyaluronic Acid Materials Support?
BOC Sciences offers HA materials widely used in the pharmaceutical, cosmetics, and food industries. In the pharmaceutical field, HA is used in ophthalmic surgery, joint lubrication, and wound healing. In cosmetics, it serves as a highly effective moisturizing and anti-aging ingredient. In the food industry, HA is used as a dietary supplement to improve joint health and skin hydration. We also provide customized HA solutions tailored to client needs, supporting the entire process from research and development to production.
Cosmetics and Skincare
HA is extensively used in cosmetics and skincare products due to its strong moisturizing properties, helping to retain skin moisture and reduce dryness and fine lines. It also promotes skin repair, enhances elasticity, and adds radiance, making it a common ingredient in anti-aging formulations.
Drug Delivery Systems
As a carrier in drug delivery systems, HA offers excellent biocompatibility and hydration properties, enabling controlled drug release and improved bioavailability. It plays a crucial role in delivering treatments for ophthalmic diseases and arthritis.
Joint Health Supplements
In the field of joint health, HA is used as a supplement to help maintain joint lubrication and alleviate pain and stiffness caused by arthritis. By enhancing the viscosity of synovial fluid, it promotes joint mobility and flexibility.
Biomedical Applications
HA is used in biomedical applications such as wound healing and tissue engineering due to its ability to accelerate cell migration and repair processes. It is widely applied in burn treatment, skin regeneration, and post-surgical recovery.
Ophthalmic Applications
In ophthalmology, HA is primarily used in the treatment of dry eye syndrome and ophthalmic surgeries. It helps maintain eye surface moisture, reduces post-surgical inflammation, and is a key ingredient in eye drops and ocular lubricants.
Plastic Surgery
HA is extensively applied in plastic surgery, particularly in joint injection treatments to relieve joint inflammation and pain. By lubricating joints and promoting cartilage repair, it enhances patients' mobility and overall quality of life.
Aesthetic Injections
HA is a commonly used component in modern aesthetic medicine, often used for wrinkle filling, facial volumization, and contouring. As a dermal filler, it effectively reduces facial wrinkles, making the skin appear smoother and firmer.
Food Industry
In the food industry, HA is used as a health supplement and functional food additive to boost immunity and promote skin health. It is also included in pet joint health supplements to improve joint function.
FAQs
How is hyaluronic acid prepared?
Hyaluronic acid is typically prepared by extraction from animal tissues (such as rooster combs or bovine eyes) or through microbial fermentation. The microbial fermentation method employs specific bacteria (such as Streptococcus or Escherichia coli) cultured under optimal conditions to produce hyaluronic acid, which is then purified and refined to obtain a high-purity product.
How to prepare a hyaluronic acid solution?
To prepare a hyaluronic acid solution, a specific amount of hyaluronic acid powder or granules is added to a suitable volume of sterile water. The mixture is stirred slowly to ensure complete dissolution. The concentration of hyaluronic acid can be adjusted as needed, and pH regulators may be used to modify the solution's acidity or alkalinity.
How is hyaluronic acid fermented?
The fermentation process of hyaluronic acid utilizes genetically engineered microorganisms (such as Escherichia coli or lactic acid bacteria). These microorganisms are cultured in a nutrient-rich medium, leveraging their metabolic pathways to synthesize hyaluronic acid. After fermentation, the final product is extracted through separation, purification, and refinement.
Can hyaluronic acid be synthesized?
Hyaluronic acid can be obtained through both chemical and biological synthesis methods. Chemical synthesis involves complex chemical reactions, whereas biological synthesis relies on microbial fermentation. The latter is more commonly used in industrial production due to its efficiency and ability to yield high-purity hyaluronic acid.